CRUDE GLYCEROL UTILISATION IN MONOLAURIN PRODUCTION USING IMMOBILISED Rhizomucor miehei LIPASE: OPTIMISATION AND THERMODYNAMICS STUDY
نویسندگان
چکیده
منابع مشابه
Structure-Guided Modification of Rhizomucor miehei Lipase for Production of Structured Lipids
To improve the performance of yeast surface-displayed Rhizomucor miehei lipase (RML) in the production of human milk fat substitute (HMFS), we mutated amino acids in the lipase substrate-binding pocket based on protein hydrophobicity, to improve esterification activity. Five mutants: Asn87Ile, Asn87Ile/Asp91Val, His108Leu/Lys109Ile, Asp256Ile/His257Leu, and His108Leu/Lys109Ile/Asp256Ile/His257L...
متن کاملImmobilization of Rhizomucor miehei Lipase on High Density Polyethylene
Immobilization of Lipase produced from Rhizomucor miehei on HDPE fine powder was investigated. As compared to an aqueous system, immobilization in a non-aquous organic medium such as n-hexane was not successful and caused enzyme denaturation. Prewetting the support with ethanol increased the immobilized protein and enzyme activity as much as 31% and 34%, respectively. The maximum immobilized a...
متن کاملEnantioselectivity of recombinant Rhizomucor miehei lipase in the ring opening of oxazolin-5(4H)-ones.
Enantioselectivity of enzyme catalysis is often rationalized via active site models. These models are constructed on the basis of comparing the enantiomeric excess of product observed in a series of reactions which are conducted with a range of homologous substrates, typically carrying various side chain substitutions. Surprisingly the practical application of these simple but informative 'pock...
متن کاملImmobilised cells of Pachysolen tannophilus yeast for ethanol production from crude glycerol.
Screening among naturally occurring yeast strains of Pachysolen spp. that are capable of producing ethanol from glycerol under aerobic conditions identified the most active culture, P. tannophilus Y -475. Conversion of glycerol by this producer immobilised in poly(vinyl alcohol) cryogel resulted in a 90% yield of ethanol relative to the theoretical limit. The maximum rate of alcohol accumulatio...
متن کاملEnantioselective transacetylation of (R,S)-β-citronellol by propanol rinsed immobilized Rhizomucor miehei lipase
BACKGROUND Use of enzymes in low water media is now widely used for synthesis and kinetic resolution of organic compounds. The frequently used enzyme form is the freeze-dried powders. It has been shown earlier that removal of water molecules from enzyme by rinsing with n-propanol gives preparation (PREP) which show higher activity in low water media. The present work evaluates PREP of the lipas...
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ژورنال
عنوان ژورنال: Journal of Oil Palm Research
سال: 2019
ISSN: 1511-2780
DOI: 10.21894/jopr.2019.0054